Poster Presentations
Day 1, June 10（Wed.） Room P (5F 501+502)
- 1P-44
Development of a Mass Spectrometric Method Based on Multi-Mode Laser Ablation Sampling with AC Corona Discharge Ion Source and Its Application to Food Imaging
(1BioChromato, 2Univ. Tokyo)
oHaruo Shimada1, Hsin Khoo1, Ai Yamashita1, Takafumi Hirata2
Mass spectrometry imaging (MSI) is widely used to visualize the spatial distribution of chemical components in biological tissues and food materials. However, conventional MSI techniques often require complicated sample preparation, such as matrix deposition, and are typically performed under vacuum. In addition, raster scanning is commonly used for irradiation, which further increases the measurement time.
In this study, we developed a mass spectrometric system combining femtosecond laser ablation (LA) with a galvanometer scanner and a proximity AC corona discharge ion source for rapid analysis under atmospheric pressure. The femtosecond galvanometer laser enables highly flexible irradiation, with scanning speeds ranging from several μm s-1 to several hundred mm s-1. In addition to conventional raster scanning, a “hollow-out irradiation” method was introduced, allowing laser exposure within arbitrarily shaped regions.
Aerosols generated by laser ablation are transported through a heated line and ionized by the AC corona discharge source. Brown rice and hop samples were analyzed as applications. Raster scanning enabled imaging of lipid and sugar distributions in brown rice, whereas hollow-out irradiation allowed rapid acquisition of localized spectra from lupulin glands in hop samples.