Program

Poster Presentations

Day 1, June 10（Wed.）　　Room P (5F 501+502)

1P-40

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Performance Evaluation of a Wide-Target Metabolome Analysis Kit for Plasma Metabolomics

(¹Tohoku Univ., ²Tohoku Univ., ³Tohoku Univ.)
^oEiji Hishinuma^1,2, Naomi Matsukawa², Ikuko Motoike^2,3, Seizo Koshiba^1,2, Masayuki Yamamoto^1,2, Kengo Kinoshita^1,2,3

Metabolomics enables comprehensive profiling of metabolites and reflects human phenotypes influenced by genetic and environmental factors. Although it has contributed to biomarker discovery for various diseases, clinical applications remain limited because reference values for many metabolites are not well established and analytical variability can hinder data comparability. Recent advances in mass spectrometry have enabled stable targeted analyses of hundreds to thousands of metabolites, and wide-targeted metabolomics kits allowing absolute quantification are increasingly used. The Tohoku Medical Megabank Organization has conducted large-scale plasma metabolomics studies using the MxP Quant 500 kit to generate reliable public datasets. Recently, the expanded MxP Quant 1000 kit, which enables absolute quantification of 1,233 metabolites across 49 classes, was introduced. In this study, we evaluated its analytical performance using human plasma, mouse plasma, and cancer tissue extracts analyzed by LC–MS/MS. Multivariate analysis distinguished sample types, and more than 1,000 metabolites were quantified with good reproducibility, demonstrating its utility for high-throughput biomarker discovery.

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