Poster Presentations
Day 1, June 10（Wed.） Room P (5F 501+502)
- 1P-38
Characterizing Nucleic Acid Payloads in Lipid Nanoparticles and Viral Vectors via Charge Detection Mass Spectrometry
(1Nihon Waters, 2Waters)
oEtsuko Yada1, Rebecca J D'Esposito2, Ying Qing Yu2, Kenji Hirose1
The characterization of nucleic acid payloads in delivery systems like lipid nanoparticles (LNPs) and adeno-associated viruses (AAVs) is essential for ensuring product quality and efficacy. Conventional techniques often struggle with the inherent heterogeneity and large size of these complexes. Charge Detection Mass Spectrometry (CDMS) addresses these challenges by enabling direct, single-particle mass and charge measurements of intact nucleic acids. In this study, we utilized CDMS to characterize payloads from LNP and AAV formulations, providing high-resolution data on size distribution and structural heterogeneity. Our results demonstrate that CDMS provides robust, quantitative assessments of genome integrity, clear differentiating between full-length, truncated, and degraded species without the need for amplification or fragmentation. These findings establish CDMS as a powerful, complementary tool for monitoring payload quality in the development and manufacturing of gene therapies and mRNA-based therapeutics.