Program

Poster Presentations

Day 1, June 10（Wed.）　　Room P (5F 501+502)

1P-21

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Development of Single-Cell Mass Spectrometry Imaging Using Oxygen Attachment Dissociation-Tandem Mass Spectrometry and Tapping-Mode Scanning Probe Electrospray Ionization

(UOsaka)
^oTasuku Kato, Takao Yasuda, Yoichi Otsuka, Michisato Toyoda

We developed a molecular surface modification method to improve long-term probe stability in tapping-mode scanning probe electrospray ionization (t-SPESI) for single-cell mass spectrometry imaging (MSI). Surface coating with triethoxy(pentafluorophenyl)silane suppressed time-dependent contamination of the probe tip. Coupling t-SPESI with oxygen attachment dissociation (OAD)-MS/MS enabled layer-specific lipid visualization and discrimination of n-7 and n-9 positional isomers of PC 34:1 in mouse cerebellum.

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