Program

Poster Presentations

Day 1, June 10（Wed.）　　Room P (5F 501+502)

1P-14

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Lipidomic profiling of AADAC-overexpressing VSMCs for Biomarker Discovery in Atherosclerosis

(¹Teikyo Univ., ²Tohoku Univ., ³Teikyo univ., ⁴Tohoku Univ.)
^oShingo Wakabayashi¹, Kyohei Morita², Arisa Ishii³, Tomoko Fukuuchi¹, Noriko Yamaoka¹, Takafumi Toyohara², Daisuke Saigusa^1,4

Atherosclerosis underlies fatal diseases such as myocardial infarction and stroke and is strongly associated with diabetes and lipid metabolic disorders. However, direct molecular analysis of human vascular tissues remains difficult. In this study, we established an in vitro model using AADAC-overexpressing human iPSC-derived vascular smooth muscle cells to identify lipid biomarkers reflecting early atherosclerotic changes. Lipids from culture supernatants and extracellular vesicles (EVs) were analyzed by UHPLC/FTMS-based lipidomics. Significant alterations were observed in several lipid species, including PC (34:1), PC (O-36:2), Cer (d38:6), and TG (45:0). Notably, TG (45:0) decreased in an AADAC expression-dependent manner in both supernatants and EVs, suggesting its potential as an early lipid biomarker of atherosclerosis.

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