Fundamental Sessions
Day 1, June 10（Wed.） 16:06-16:24 Room D (5F 511+512)
- 1D-O2-1606（2P-05）
Saliva-extracellular vesicles in oral verrucous hyperplasia: using proteomics and metabolomics to develop prognostic biomarkers
(1NDMC, 2TMU)
oShih-Chi Huang1,2, Shih-En Tang1, I-Lin Tsai2
Oral verrucous hyperplasia (OVH) is a critical oral potentially malignant disorder, yet biomarkers predicting its progression to oral cancer remain elusive. This study aimed to identify non-invasive biomarkers by isolating extracellular vesicles (EVs) from the saliva of OVH patients. Using liquid chromatography-mass spectrometry (LC-MS), we performed parallel proteomic and metabolomic analyses on EV fractions. Proteomic profiling identified three candidate proteins—Cystatin S, Cornulin, and Fibrinogen Gamma Chain—that effectively differentiated progressive from non-progressive OVH. Complementary metabolomic analysis revealed significant dysregulation in arachidonic acid metabolism and steroid biosynthesis pathways. Despite challenges related to low salivary EV yields, this dual-omics approach highlights specific EV-derived proteins and metabolites as promising indicators for the early risk assessment of oral squamous cell carcinoma (OSCC). These findings provide a vital foundation for future multi-omics integration to validate progression biomarkers.