Luncheon Seminar
Day 1, June 10（Wed.） 12:40-13:40 Room C (4F 413)
- 1C-L-1240
Development of a Highly Precise and Sensitive Single-cell Mass Spectrometry Method for Organelle Metabolomics
(Meijo Univ.)
oHajime Mizuno
There are increasing demands to study the molecular and cellular behavior of a single cell precisely because the current knowledge in life science was obtained using multiple cells, with little information on the localization or environment of cells. We developed a “Live Single-cell Mass Spectrometry” method, which allows direct analysis of xenobiotics and endogenous compounds in a living cell. In this study, we applied this method to analyze organelle-localized metabolites in single cells. The method is consists of two steps: (1) cellular contents are sampled from living cells using a platinum-coated glass capillary (i.d. 3 m) under confocal microscopic observation with an automated cell sampling device (SS2000, Yokogawa Electric Corporation), and (2) the capillaries are directly subjected to static nano-electrospray ionization of the sampled cellular contents such as cytosol or organelles for mass spectrometry. For mass spectrometry, triple quadrupole (QQQ) and quadrupole time-of-flight (Q-TOF) mass spectrometers were used for targeted and untargeted metabolite analysis, respectively. Furthermore, sample normalization and derivatization methods were developed to enable highly precise and sensitive analysis of organelle metabolites.