Symposium Sessions
Day 1, June 10（Wed.） 16:20-16:40 Room B (4F 411+412)
- 1B-S2-1620
Development of a Next-Generation Fully Automated Lipidome Analysis Platform
(1Osaka Univ., 2Shimadzu, 3Niigata Univ., 4Kazusa DNA Res. Inst.)
oTakeshi Bamba1, Hirofumi Fujimoto2, Kenta Matsumoto2, Masaki Yamada2, Kohta Nakatani3, Kazutaka Ikeda4
Recent advances in mass spectrometry have enabled comprehensive measurement of lipid molecules involved in biological regulation and disease. However, large-scale lipidomics studies still face challenges, including reliance on operator expertise and difficulty in maintaining consistent performance across laboratories.
To address these issues, we are developing a fully automated lipidomics platform under the Council on Competitiveness-Nippon initiative “Lipid Center for Lipidomics,” in collaboration with Osaka University, the Kazusa DNA Research Institute, and Shimadzu Corporation. The goal is to establish an integrated infrastructure for comprehensive lipid analysis with minimal manual intervention.
Lipid metabolites are classified into three groups based on physicochemical properties: major structural lipids, oxylipins, and cholesterol-related metabolites. Dedicated LC/MS and SFC/MS methods were integrated with robotic sample-preparation modules to create a seamless workflow from extraction to analysis. The automated system enables coordinated operation between preparation and MS measurement, improving efficiency and reducing variability.
Initial evaluation demonstrated stable performance and reproducibility comparable to conventional workflows. Current work focuses on quality control and data standardization for long-term and multi-site studies. This platform provides a foundation for high-throughput, reproducible lipidome analysis and supports clinical and biomedical applications.