演題概要

ポスター発表

第3日　5月17日（水）　　P会場（ホワイエ，会議室1004-1007）

3P-48　　PDF

Detection of Different Distributions of Acetaminophen and its Metabolite at 10 µm Spatial Resolution by Atmospheric Pressure Matrix-assisted Laser Desorption Ionization Imaging Mass Microscope

(¹Hamamatsu Ikadaigaku・ ²Preppers・ ³Int. Mass Imaging Center)
^oMamun, Md Al^1,2・ Rahman, Muedur¹・ Sakamoto, Takumi^1,2・ Islam, Ariful^1,2・ Oyama, Soho¹・ Nabi, Md Mahamodun¹・ Sato, Tomohito¹・ Kahyo, Tomoaki¹・ Takahashi, Yutaka^1,2・ Setou, Mitsutoshi^1,3

Matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) and desorption electrospray ionization (DESI)-MSI has attracted much attention in drug distribution studies. However, the typical highest spatial resolution of MALDI-MSI and DESI-MSI is 50 µm which is not enough to resolve complex tissues (brain, kidney, etc). Moreover, many drugs in tissues are poorly detected or undetectable by vacuum MALDI-MSI and DESI-MSI. For example, acetaminophen (APAP) and one of its major metabolites, APAP-Cysteine (APAP-CYS), cannot be detected by vacuum MALDI-MSI without derivatization. To this end, we aim at the high spatial mapping of this drug and its metabolite in tissues. For this purpose, we employed a newly developed atmospheric pressure-MALDI imaging mass microscope (iMScope™ QT, Shimadzu, Japan). Interestingly, iMScope QT was capable of imaging native APAP and APAP-CYS in mice kidneys at 25 µm and 10 µm spatial resolution. APAP was accumulated in the renal pelvis, while APAP-CYS showed characteristic distributions in the outer medulla and renal pelvis. Our study showed the capability of iMScope QT to detect APAP and APAP-CYS at high spatial resolution (up to 10 µm) which is fairly higher compared to other MALDI-MSI and DESI-MSI instruments.