演題概要

ポスター発表

第3日　5月17日（水）　　P会場（ホワイエ，会議室1004-1007）

3P-44　　PDF

Rapid Mapping of Imipramine, Chloroquine and Their Metabolites in Mice by AP-MALDI Mass Spectrometry Imaging Combined with Product-Ion Analysis

(¹HUSM・ ²MSI Center, HUSM・ ³Shimadzu)
^oIslam, Ariful¹・ Zhai, Qing¹・ Rahman, Md.¹・ Takahashi, Yutaka^1,2・ Sakamoto, Takumi¹・ Mamun, Md.¹・ Ogata, Koretsugu³・ Kasamatsu, Satoshi³・ Kahyo, Tomoaki^1,2・ Setou, Mitsutoshi^1,2

Introduction: In this study, we applied a newly developed atmospheric pressure matrix-assisted laser desorption ionization mass spectrometry imaging (AP-MALDI-MSI) known as iMScopeTM QT (Shimadzu, Japan) for rapid mapping of imipramine, chloroquine, and their metabolites in mice.
Methods: AP-MALDI-MSI data were acquired rapidly (up to 32 pixels/s) by applying iMScope QT from 10 μm sections of the kidney, brain, and liver of imipramine and chloroquine-treated mice. Detected drugs and their metabolites were confirmed by product ion analysis.
Results and Discussion: Applying iMScope QT, we revealed the localization of imipramine, chloroquine, and their metabolites in mice. We observed that imipramine and its metabolites were highly accumulated in the upper medulla of mice kidneys, whereas chloroquine and its metabolites were significantly accumulated in the pelvis and inner medulla of mice kidneys. Additionally, a higher accumulation of imipramine was noted in the hindbrain, midbrain, thalamus, hypothalamus, and septum of the mice brains, and a notable accumulation of chloroquine was observed in the lateral ventricle, 4th ventricle, and fornix of the mice brains. These drugs and their metabolites were also successfully detected in the mice's liver by iMScope QT. Our study showed the potentiality of iMScope QT for rapid imaging of small drugs.