ポスター発表
- 第3日 5月17日（水） P会場（ホワイエ，会議室1004-1007）
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3P-34 PDF
超臨界流体クロマトグラフィー／中真空化学イオン化質量分析法の開発
Online coupling of supercritical fluid extraction (SFE) and supercritical fluid chromatography (SFC) with medium vacuum chemical ionization (MVCI) mass spectrometry (MS) has been investigated. This novel analytical method has great potential to quantitate lipophilic small molecules contained in an extremely small sample, such as a cell. Obtained lower limit of quantitation (LLOQ) for arachidonic acid and arachidic acid were 1.2 and 2.7 fmol, respectively. Sixteen free fatty acids were quantified from 100 nL human pool serum, with a repeatability of 0.1 to 16% of relative standard deviation.