ポスター発表
- 第3日 5月17日（水） P会場（ホワイエ，会議室1004-1007）
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3P-17 PDF
各成熟段階におけるバナナのガンマアミノ酪酸とグルタミン酸脱炭酸酵素反応に関する研究
Banana is the fourth most consumed crop in the world and is very popular due to its high economic value and health benefits. Banana is a climacteric fruit that continues to ripen after harvesting, and it has been reported that endogenous substances in bananas change significantly during the ripening process. γ-Aminobutyric acid (GABA) is abundant in bananas and is reported to have a blood pressure lowering effect. Therefore, bananas are gaining popularity as a functional food.
Glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the biosynthesis of GABA from glutamate. It has been suggested that GAD gene expression is involved in ethylene biosynthesis, which is essential for banana ripening, but changes in the localization of the GAD enzyme reaction during the ripening stage have not been reported. Mass spectrometry imaging (MSI) is a technique to visualize the distribution of target components by combining positional information from optical images with mass spectrometry results. Recently, MSI has been applied to obtain localization information of enzymatic reactions. The objective of this study is to investigate the relationship between the localization of endogenous GABA and GAD reactions at each ripening stage using MSI technology.