演題概要

若手研究者セッション

第3日　5月17日（水）　10:00～10:15　C会場（会議室1009）

3C-O-1000（2P-17）　　PDF

マシンガンプロテオミクスによる空間プロテオームイメージング

(¹京大院薬・ ²理研IMS・ ³慶大薬・ ⁴医薬基盤健栄研)
^o富岡郁那¹・ 富岡亮太¹・ 小形公亮¹・ 森一晟^2,3・ 有田誠^2,3・ 今見孝志²・ 杉山直幸¹・ 石濱泰^1,4

Spatial proteomics is a promising technique to profile protein expression in different tissue regions. While current antibody-based spatial mapping methods are sensitive, selective, and high-resolution, they can quantify only pre-defined proteins. In contrast, shotgun proteomics enables untargeted protein quantitation and has the potential to break through the limitation of antibody-based methods. However, typical shotgun proteomics requires several hours per nanoLC/MS/MS measurement, making the combination with MS imaging impractical. We have therefore developed a “machine-gun proteomics” platform that is more than 100 times faster than typical shotgun proteomics, at only 1.4 minutes per sample. Our method could obtain 1,000 sections within a day, allowing us to obtain not only 2D, but 3D spatial proteome expression profiles within a feasible time.