演題概要

若手研究者セッション

第3日　5月17日（水）　09:30～09:45　C会場（会議室1009）

3C-O-0930（1P-03）　　PDF

LC/MSによる肺腺がん患者血漿由来細胞外小胞を用いたバイオマーカー探索

(¹帝京大薬・ ²帝京大医・ ³がん研究会・ ⁴東北大メガバンク・ ⁵ベーリンガー・ ⁶帝京医溝口)
^o岩崎裕子¹・ 本田健²・ 植田幸嗣³・ 福内友子¹・ 山岡法子¹・ 菱沼英史⁴・ 松川直美⁴・ 富樫亮⁵・ 松谷哲行⁶・ 関順彦²・ 三枝大輔^1,4

Extracellular Vesicles (EVs) contain metabolites, such as nucleotides and lipid species, and transport them playing as the intracellular mediators. However, it is hard to detect these metabolites due to the limit of detection. We firstly established the analytical procedure for metabolic profiling of EVs by means of UHPLC-MS/MS and UHPLC-FTMS analysis. Then, we examined to identify the metabolic variation in plasma EVs derived from lung adenocarcinoma patients and healthy volunteers. As results, cysteine and cystine were significantly higher in the EVs from cancer patient, and observed the variation of lipid species, such as triglycerides and phosphatidylcholines consist with polyunsaturated fatty acids. We surmise that the oxidative stress related metabolites in EVs might be correlated with the progression of cancer, and the mechanism should be elucidated in the future study.