基盤セッション
- 第3日 5月17日（水） 10:27～10:45 B会場（会議室1202）
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3B-O-1027 PDF
イオンモビリティー質量分析法によるピリジルアミノ化糖鎖の異性体識別
Determination of glycan structures is key in understanding function relationships of glycans and glycoconjugates including biopharmaceuticals. Mass spectrometry (MS) plays a major role in analyzing glycan structures owing to its high sensitivity and mass resolution. A central issue in glycan mass analysis is the ambiguity of structural assignments due to the heterogeneity and complexity of glycan structures. The complexity of glycan mass analysis is mainly related to structural isomers. Glycan isomerism is caused by differences in anomericity (α/β), linkage pattern (1–2/1–3/1–6 etc.), and composition (Glc/Gal/Man and GlcNAc/GalNAc, etc.). We previously proposed a method for identification of N-glycan structures by ultraperformance liquid chromatography-connected ion mobility mass spectrometry (UPLC/IM-MS). We here examine 71 pyridylaminated (PA-) N-linked oligosaccharides including isomeric pairs. A dataset on collision drift times, retention times, and molecular mass was collected for these PA-oligosaccharides. For standardization of the observables, LC retention times were normalized into glucose units (GU) using pyridylaminated α-1,6-linked glucose oligomers as reference, and drift times in IM-MS were converted into collision cross sections (CCS). CCS correlates with the 3D structure of glycans. From these, we propose a strategy for practical structural analysis of N-linked glycans based on the database of m/z, CCS, and GU.