演題概要

基盤セッション

第3日　5月17日（水）　09:33～09:51　A会場（特別会議場）

3A-O-0933　　PDF

質量分析スクリーニングを活用したクロマトグラフ用キラル固定相開発

(¹大阪産業技術研・ ²阪工大・ ³阪大産研)
^o靜間基博^1,2・ 李翔太²・ 松崎剛³・ 朝野芳織³・ 川野真太郎¹・ 佐藤博文¹・ 村岡雅弘²・ 鈴木健之³

As inulin which is one of polysaccharides has polyoxyethylene structure as the main chain, it makes noncovalent complex with cationic species via charge-dipole electrostatic intermolecular interaction. The chiral discrimination ability of the oligomer mixture of inulin derivative (methyl, benzyl, acetyl, and benzoyl) toward several 2-propyl ester of protonated chiral amino acid was evaluated using fast atom bombardment mass spectrometry coupled with the isotopically labeled guest method which was proposed by Sawada et al. Permethylated inulin among the inulin derivatives showed high chiral discrimination ability toward given chiral cations. Permethylated inulin was fixed on silica gel to use as chiral stationary phase in liquid chromatography and the chiral separation factor toward chiral ammonium ion was examined. The separation factors values showed linear relationship to the chiral discrimination ability in the FABMS screening.