演題概要

ポスター発表

第2日　5月16日（火）　　P会場（ホワイエ，会議室1004-1007）

2P-47　　PDF

タンデム質量分析計でのDIA測定によるリボヌクレアーゼ消化mRNA の自動評価アルゴリズム

(¹三井情報・ ²理研CSRS・ ³都立大・ ⁴Waters - Milford・ ⁵Waters - Wilmslow・ ⁶Waters - S.A.S)
^o松原佑記¹・ 伊藤朱里¹・ 横井靖人¹・ Fox, Jonathan⁵・ Gaye, Maissa⁴・ Fredette, Joe⁴・ Bechade, Guillaume⁶・ Lauber, Matthew⁴・ 小池仁美²・ 延優子³・ 田岡万悟³・ 中山洋²

The demand for the characterization of modified messenger (m)RNA is increasing with the development of mRNA therapeutics. An effective experimental technique for mRNA characterization is digestion with sequence-specific ribonuclease, such as RNase T1, followed by analysis of the digest by liquid chromatography (LC)-tandem mass spectrometry (MS/MS). Although digests are preferably analyzed one by one by data-dependent MS/MS acquisition (DDA), in such experiments some nucleotide ions are often missed by chance. In contrast, data-independent MS/MS acquisition (DIA) experiments allow all detected nucleotide ions to be captured in a one-shot analysis; however, there is no software tool for DIA data of RNA. Here, we propose a new algorithm for the characterization of long, modified RNAs using DIA MS/MS data.