ポスター発表
- 第2日 5月16日（火） P会場（ホワイエ，会議室1004-1007）
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2P-09 PDF
MIMICS: 統合マルチオミックス解析により宿主-微生物の相互作用を調べるための体外共培養装置
Akkermansia muciniphila has been identified as a potential next-generation probiotic that could enhance host health and prevent metabolic diseases. However, the biological interactions between A. muciniphila and human intestinal epithelial cells (Caco-2) for use in biotherapeutics have not been extensively studied. To address this knowledge gap, we developed an in vitro culture device named MIMICS, which simulates the human intestinal environment and enables multi-omics analysis. Our study demonstrated that both A. muciniphila and human intestinal epithelial cells remained viable for 12 hours after co-culture in MIMICS. Subsequently, we conducted transcriptomic and proteomic analyses to observe the changes in intestinal epithelial cells caused by A. muciniphila treatment, including changes in cell-cell junctions, immune response, and mucus secretion. Our findings are consistent with those reported in previous in vivo studies. Furthermore, our results revealed that A. muciniphila activates glucose and lipid metabolism in intestinal epithelial cells, leading to an increase in ATP production. Overall, our study suggests that A. muciniphila can activate metabolism in intestinal epithelial cells and that the developed MIMICS system is a useful tool for evaluating the effects of bacteria on intestinal epithelial cells in vitro.