ポスター発表
- 第2日 5月16日（火） P会場（ホワイエ，会議室1004-1007）
-
2P-07 PDF
ナノLC-MS/MSを用いたターゲットプロテオミクスのLipomyces starkeyiへの適用
Targeted proteomics using nano-LC-MS/MS was applied to the oleaginous yeast Lipomyces starkeyi. The protein extraction method was optimized by using a multi beads homogenizer. MRM assay methods including 2,288 transitions were also constructed to measure the expression levels of 107 enzyme proteins. The targeted proteomic analysis of the central metabolism-related enzymes revealed that those expression levels in the lipid production phase were significantly lower than that of the exponential growth phase.