ポスター発表
- 第2日 5月16日（火） P会場（ホワイエ，会議室1004-1007）
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2P-04 PDF
LC-MS/MSを用いたマルチオミクス解析によるFaecalibacteirum prausnitziiによるプレバイオティックイヌリン消化機構の解明
The gut bacteria Faecalibacterium prausnitzii is known for its anti-inflammatory properties and positive effects on intestinal health. Prebiotic inulin can increase the presence of F. prausnitzii in the gut. Furthermore, consuming an inulin-rich diet has been shown to stimulate butyrate production in mouse models, and there is mounting evidence that it may improve intestinal health in vivo. However, the precise mechanism underlying these inulin effects in the gut is unclear. Here, we applied LC-MS/MS based multiomics approaches to identify biological and enzymatic mechanisms of F. prausnitzii involved in the selective digestion of inulin. Certain proteins that break down inulin-type fructans, such as β-fructosidase and amylosucrase, were found to be more active when inulin was present, according to cell protein and metabolite analysis. Additionally, fructan hydrolases in the cell broke down inulin-type fructans absorbed by fructan ATP-binding cassette transporters. The study also observed that the fructose phosphotransferase system transporter improved fructose uptake, allowing F. prausnitzii to use fructose produced during the breakdown of inulin-type fructans as an energy source for inulin utilization. Taken together, this study provided molecular insights regarding the metabolism of F. prauznitzii for inulin, which stimulates the growth and activity of the beneficial bacterium in the intestine.