ポスター発表
- 第2日 5月16日（火） P会場（ホワイエ，会議室1004-1007）
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2P-03 PDF
統合的マルチオミクスアプローチによるAkkermansia Muciniphilaの脂肪細胞における抗脂肪生成および抗脂肪誘発効果の特性評価
Akkermansia muciniphila is a next-generation probiotic and a potential biotherapeutic for improving obesity, diabetes, and metabolic diseases. Although many positive effects have been associated with obesity, previous studies have only reported changes in cell phenotype or markers associated with obesity. This study used multiomics approaches to investigate the molecular mechanism involved in treating obesity with A. muciniphila in 3T3-L1 cells. The results showed that treatment with A. muciniphila cell lysate suppressed lipid accumulation in 3T3-L1 cells at the cellular level and downregulated the mRNA expression of proteins involved in adipogenesis and lipogenesis. Proteomic analysis revealed that A. muciniphila decreased the expression of proteins involved in fat cell differentiation, fatty acid metabolism, and energy metabolism in adipocytes. Additionally, A. muciniphila reduced the level of metabolites related to glycolysis, the TCA cycle, and ATP in adipocytes. The study also found that A. muciniphila upregulates the expression of SERPINA3G, a serine protease inhibitor, which inhibits lipogenesis in adipocytes. These findings suggest that the cellular components of A. muciniphila could be promising biotherapeutics for obesity. Overall, this study provides new insights into the potential mechanisms of A. muciniphila in obesity and highlights its potential as anti-lipogenic agents.