演題概要

シンポジウムセッション

第2日　5月16日（火）　14:40～15:05　B会場（会議室1202）

2B-S-1440　　PDF

分子レベル同位体比計測の最先端技術：地球物質循環と生態環境解析のための有機化合物の炭素・窒素・硫黄同位体比分析法

(海洋機構)
^o小川奈々子・ 髙野淑識・ 大河内直彦

The research method using molecular-level isotope analysis (Compound-Specific Isotope Analysis, CSIA) of organic compounds was originated in the 1960s and has since been recognized and applied in various fields of science. In this talk, we will discuss the "state-of-the-art molecular-level carbon & nitrogen isotope analysis technology" we have been developing, based on optimized pretreatment, compound purification process with HPLC (without isotope ratio alterations), and nano-EA/IRMS isotope analysis. In particular, recent advances in ¹³C-, ¹⁵N-, and ³⁴S-CSIA of the compounds involved in central metabolism and derived functional compounds such as amino acids and nucleobases will be highlighted. With examples of our latest results obtained from biogeochemical materials such as aquatic organisms, suspended particles, and sediments, we will also discuss the technical details of our methods, their potential benefits, and challenges to overcome in our perspectives. It is important to note that the refined analytical methods of the ¹³C-CSIA provide new perspectives in an interdisciplinary area of ¹⁴C-CSIA