シンポジウムセッション
- 第2日 5月16日（火） 15:09～15:27 A会場（特別会議場）
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2A-S-1509 PDF
フェロトーシス関連揮発性代謝物を標的とした呼気バイオプシー技術の創出
Human exhaled air contains a large number (>1000) of volatile organic compounds (VOCs). Since individual VOCs are produced and released through specific metabolic reactions, they have attracted attention as novel non-invasive biomarkers to monitor health status and disease onset.
Lipid peroxidation (LPO) has been considered one of the major origins of biological VOCs. Volatile oxidized lipids (VOLs), such as short-chain hydrocarbons and aldehydes, are released from biological lipids by oxidative fragmentation. In addition, since LPO has been recognized as an important contributor in iron-dependent cell death, ferroptosis and the pathogenesis of ferroptosis-related diseases, the analytical technologies for VOLs will enable futuristic medical diagnosis to predict disease risk from "exhaled breath".
However, the detailed generation mechanisms of VOLs have not been well understood. Therefore, the profiles of biological VOLs generated in ferroptosis have been unclear. To address this challenge, we here constructed the structural library of VOLs and also identified ferroptosis-specific volatile metabolites. Furthermore, we applied our developed library to the comprehensive analysis of biological VOLs released from nonalcoholic steatohepatitis (NASH)-model mice, and found that the levels of several VOLs, especially ω-6 PUFA derived VOLs, in mouse biogas were elevated with NASH progression. Our developed methodologies are expected to be innovative tools for non-invasive detection of LPO-related diseases from exhaled breath.