シンポジウムセッション
- 第2日 5月16日（火） 09:15～09:35 A会場（特別会議場）
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2A-O-0915 PDF
質量によるバイオ医薬品タンパク質の特性解析
The macromolecular medical drugs produced using biotechnology are called biopharmaceuticals. Typical active ingredients of biopharmaceuticals are proteins, and their properties depend on sequences, structures and post-trans modifications. In addition, most of proteins interact with other molecules to perform their functions. Therefore, the characterizations of proteins are important to ensure the safety and efficacy of biopharmaceuticals. In this talk, I will introduce the application of hydrogen/deuterium exchange mass spectrometry (HDX-MS) and crosslinking mass spectrometry (XL-MS) on the interaction between immunoglobulin G1 (IgG1) and Fc gamma receptor IIIa (FcγRIIIa). Combined HDX-MS and XL-MS revealed that the Fab portion IgG1 contributes the interaction with FcγRIIIa in addition to Fc portion. Additionally, the characterizations of adeno-associated virus vectors (AAV) which are leading vectors for gene therapy using mass photometry (MP) will be introduced. MP is based on interference scattering microscopy to estimate the molecular weight by detecting the scattered light of each molecule adsorbed on a glass surface. MP analysis indicates that AAV releases its genome while maintaining the capsid structure, and that the temperature at which the genome is released is dependent on the genome length within the capsid. Mass analysis is a useful tool for obtaining the information about protein properties.