演題概要

ポスター発表

第1日　5月15日（月）　　P会場（ホワイエ，会議室1004-1007）

1P-41　　PDF

液体クロマトグラフィーイオンモビリティー質量分析法による血中代謝物の分析と主成分解析

(¹富山県立大・ ²富山県立大院工・ ³金沢大国基)
^o高田莉子¹・ 山川舞²・ 古関涼介¹・ 唐島成宙³・ 大坂一生^1,2

In quantitative analysis of steroids using LC/MS/MS, multiple ion monitoring (MRM) method has been used for highly sensitive detection. The MRM is targeted analysis method. Therefore, unknown and unexpected components could not be detected by MRM. Comprehensive analysis is required to elucidate various disease in metabolomics. In this study, metabolites in the serum were detected with high sensitivity and selectivity using LC/IM/MS. Steroids ions for targeted analysis and lipid ions for non-targeted analysis were observed. In addition, obtained LC/IM/MS data were statistically analyzed by principal component analysis (PCA). Healthy subjects and various hypertensive patients were classified based on steroidal differences.