演題概要

ポスター発表

第1日　5月15日（月）　　P会場（ホワイエ，会議室1004-1007）

1P-37　　PDF

感染赤血球試料からマラリア原虫由来タンパク質とヒト由来タンパク質を質量分析によって効率的かつ的確に同定する方法

(¹神戸大バイオ・ ²神戸大院医・ ³神戸大院保健・ ⁴神戸薬大)
^o吉野健一^1,2・ 面田彩馨³・ 岡田小夏³・ 竹内敦子⁴・ 入子英幸³

There are only 413 entries of Plasmodium falciparum proteins in the SwissProt (uniport_sprot.fasta, October 2022 edition)．Therefore, sequence database searches against the SwissProt database using mass spectrometry data from samples prepared from infected human erythrocytes containing malaria parasite P. falciparum-derived proteins may not identify significant proteins if the major component is a malaria parasite P. falciparum-derived protein. In addition, “false positive” results of identifying human proteins that are not major components may arise. In this presentation, we report a method for efficiently and accurately identifying P. falciparum-derived proteins in samples containing both P. falciparum-derived and human proteins.