ポスター発表
- 第1日 5月15日（月） P会場（ホワイエ，会議室1004-1007）
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1P-19 PDF
Quantitative Analysis of Multiple Areca Nut-Specific Alkaloids in Areca Nut Products from Different Countries Using LC-MS/MS
Both the areca nut (AN) and arecoline (the major areca nut-specific alkaloid, ASA) are classified as human carcinogens. Nearly 600 million people consume AN worldwide, particularly in Southern Asia (e.g., India, China and Taiwan). The AN-associated products vary greatly from country to country. It is surprising that little information is available on the ASAs contents in various AN products from different countries. In addition to arecoline, there are other important ASAs (i.e., arecaidine, guvacoline, guvacine and N-methylnipecotic acid), which have yet to be fully assessed.
Here, we measured five ASAs in various AN products from India, Myanmar, China and Taiwan using a validated LC-MS/MS method. AN products were ground and were extracted by water. The alkaloids were analyzed by a C18 analytical column, interfaced with an API 4000 triple quadrupole mass spectrometer. Our results show that arecoline is the most abundant ASA found in all the AN products (0.61-2.04 mg/g), followed by arecaidine (0.35-1.44 mg/g), guvacine (0.25-0.68 mg/g), guvacoline (0.07-0.57 mg/g) and N-methylnipecotic acid (0.07-0.57 mg/g). The contents of ASAs vary significantly from one product to another, and this could be attributed to the variations in maturity/species of AN and/or the manufacturing process.