ポスター発表
- 第1日 5月15日（月） P会場（ホワイエ，会議室1004-1007）
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1P-01 PDF
Detection of Benzene-Induced DNA Adducts in Mice Urine Using DNA Adductomic Approach
Benzene is a well-known human carcinogen that can cause leukemia and acute lymphomas. In vivo, benzene is metabolized to a variety of reactive metabolites, which can bind covalently to DNA, and subsequently initiate carcinogenesis. To date, only a few benzene-induced DNA adducts were reported in the literature. It is proposed that many benzene-induced DNA adducts have yet to be discovered. Mass spectrometry (MS) based-DNA adductomics is an emerging technique that permits a comprehensive analysis of DNA adducts. In particular, the use of high-resolution mass spectrometry (HRMS) enables the identification of exact molecular masses and further the characterization of both the known and unknown DNA adducts.
We developed a DNA adductomic approach using liquid chromatography-quadrupole/linear ion trap/orbitrap tribrid MS with data-dependent MS2 acquisition, to comprehensively detect the benzene-induced DNA adducts in mice urine after the injection of benzene. The DNA adducts associated with the benzene exposure were characterized and identified based on the accurate mass of precursor ions and the observations of specific fragments from HRMS. Our DNA adductome maps show that numerous DNA adducts were newly formed after benzene exposure (> 80 adducts). Three deoxythymidine-associated adducts and one deoxyadenosine-associated adduct are newly characterized.