シンポジウムセッション
- 第1日 5月15日（月） 14:38～15:00 A会場（特別会議場）
-
1A-S-1438 PDF
最新 HDX-MSを用いた、糖タンパク質に対するエピトープ解析の実際
Recently, it has been reported that epitope mapping of monoclonal antibody against hemagglutin was performed by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS), while glycosylation sites were uncovered due to the difficulty of detecting and quanitfying peptides containg glycans by HDX-MS1). Here, we will introduce an approach for epitope mapping on glycosylated SARS-CoV2 receptor-binding domain of S protein by the latest HDX-MS system consist of Orbitrap Eclipse, PAL and UPLC.
1) Puchades C, et al. Sci Rep. 2019 Mar 18;9(1):4735.