シンポジウムセッション
- 第1日 5月15日（月） 14:15～14:38 A会場（特別会議場）
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1A-S-1415 PDF
同重体標識アフィニティー精製-質量分析法を用いた抗HCP抗体の免疫反応プロファイリング
We evaluated the immunoreactivity profiles of eight commercial anti-host cell protein (anti-HCP) antibodies from different host animals and their antigens used for immunization by newly developed isobaric labeled affinity purification-mass spectrometry (AP-MS) method. As a result, 34 proteins with high abundance but low recovery from harvest cell culture fluid were identified. Since they are likely to be underestimated in biopharmaceutical quality assessment, the features common to these proteins were investigated. Compared to other immunoprecipitated HCP proteins, proteins exhibiting lower molecular weight (ΔMW = −14600), lower isoelectric point (ΔpI = −0.86), and lower hydrophobicity (ΔGRAVY = −0.13) were enriched. This AP-MS method provides important information for HCP control strategies using immunological methods and is expected to contribute to the development of safe biopharmaceutics.