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第3日　5月21日（金）　　P2会場（Zoom）

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質量分析法を用いた分化前後のヒト単球細胞株THP1の¹³C代謝フラックス解析

(阪大院情報)
^o本谷真奈・ 谷口赳夫・ 岡橋伸幸・ 松田史生

¹³C-metablic flux analysis (MFA) using mass spectrometry was used to investigate metabolic flux distributions in differentiated and non-differentiated THP1 monocyte leukemia cells. A time course analysis of cell numbers, medium components and ¹³C-labeling kinetics of the intracellular metabolites confirmed that ¹³C-MFA could be applied for the immune cells. Flux distributions in the central carbon metabolism showed that the NADPH regeneration rate was increased by 7-fold in differentiated THP1 cells compared with non-differentiated ones, suggesting that metabolism of differentiated THP1 was rewired for immune responses.