演題概要

ポスター発表

第3日　5月21日（金）　　P1会場（Zoom）

3P-27　　PDF

カイコで生産された糖鎖改変バイオ医薬品の糖鎖解析

(¹横市大・ ²農研機構・ ³阪大院工)
^o向武志¹・ 立松謙一郎²・ 高倉大輔¹・ 三﨑亮³・ 梶浦裕之³・ 藤山和仁³・ 瀬筒秀樹²・ 川崎ナナ¹

Using LC/MS/MS, we analyzed the glycan structure of rituximab and etanercept produced in transgenic silkworms expressing galactosyltransferase, sialyltransferase and other related enzymes. We found that sialylated and galactosyled hybrid type N-glycans are attached to the transgenic silkworm-derived glycoproteins. Our result suggests that activity of enzymes required for complex type glycan biosynthesis may be lower in silkworms compared to CHO cells.