演題概要

ポスター発表

第3日　5月21日（金）　　P1会場（Zoom）

3P-17　　PDF

LC-QTOF/MSを用いた出芽酵母実用株のノンターゲットリピドミクス

(¹阪大院情報・ ²島津・ ³阪大島津協働研・ ⁴阪大先導研)
^o小森柊花¹・ 岡橋伸幸^1,3,4・ 小倉泰郎²・ 飯田順子^2,3・ 松田史生^1,3,4

Comprehensive analysis of lipid molecules in budding yeast Saccharomyces cerevisiae, a model eukaryote organism, was performed using LC-QTOF/MS in a data-dependent acquisition (DDA) mode. 362 molecular species in 28 lipid classes in four industrial S.cerevisiae strains (laboratory yeast, wine yeast, bread yeast, and sake yeast) were successfully identified by MS-DIAL software. A clear difference in fatty acids chain length, the abundance of cardiolipin and CoQ6, and total amounts of TAG and SE was observed among the four strains, suggesting the relevance of the lipids and industrial yeast phenotypes.