ポスター発表
- 第3日 5月21日（金） P1会場（Zoom）
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3P-15 PDF
LC-MS/MSを用いた代謝物濃度測定法の拡張と出芽酵母実用株への適用
We extended the method for measuring the intracellular concentration of metabolites using LC-MS/MS. In order to expand the target compounds of the reverse labeling method, we attempted to use the LC-MS method with a PFPP column. As a result, we could measure the concentrations of 71 intracellular metabolites out of 91 metabolites that we attempted to analyze. In addition to the glycolytic system, pentose phosphate pathway, and TCA circuit intermediates, the concentrations of amino acids and 32 nucleic acid-related substances were successfully determined.