日本質量分析学会 第69回質量分析総合討論会会

演題概要

オーラルセッション

第2日 5月20日(木) 11:40~12:00 B会場(Zoom)

ヒトiPS細胞の神経分化に伴う糖タンパク質合成とN型糖鎖修飾の変化の解析

(横市大院生命医)
木村一雅浦澤貴哉小泉匠高倉大輔太田悠葵o川崎ナナ

To understand the quality attribute of human-induced pluripotent stem cells-derived neuronal cells, the changes in glycoprotein production and their N-glycosylation during neuronal differentiation was analyzed by a sequential LC/MS/MS of cellular tryptic digest, enriched glycopeptides, and deglycosylated peptides. Our results and proposed method could provide valuable information for the development of processes and quality control strategies in the development of cell products.