日本質量分析学会 第69回質量分析総合討論会会

演題概要

オーラルセッション

第2日 5月20日(木) 10:00~10:20 B会場(Zoom)

糖タンパク質の質量分析

(1産総研細胞分子2北大人獣)
o梶裕之1富岡あづさ1日尾野隆大1,2佐々木道仁2大場靖子2澤洋文2久野敦1

Antigen determining blood types like ABO-type or Lewis-type is glycans attached on protein or lipid. Thus, in case of injectable medications such as vaccine, biopharmaceuticals, or viral vector, especially they were produced from non-human organisms or their cells, immunogenicity due to glycan is requested to test strictly. As an approach to analyze glycans on protein, we developed 2 methods employing mass spectrometry. One is a method for large-scale mapping of glycosylation sites, called IGOT, and another is to assign site-specific glycan compositions of a large number of proteins or in-depth for single protein, the method is named Glyco-RIDGE. In this talk, approach and applications of the methods will be presented.