オーラルセッション
- 第2日 5月20日(木) 10:00~10:20 B会場(Zoom)
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2B-O7-1000 PDF
糖タンパク質の質量分析
Antigen determining blood types like ABO-type or Lewis-type is glycans attached on protein or lipid. Thus, in case of injectable medications such as vaccine, biopharmaceuticals, or viral vector, especially they were produced from non-human organisms or their cells, immunogenicity due to glycan is requested to test strictly. As an approach to analyze glycans on protein, we developed 2 methods employing mass spectrometry. One is a method for large-scale mapping of glycosylation sites, called IGOT, and another is to assign site-specific glycan compositions of a large number of proteins or in-depth for single protein, the method is named Glyco-RIDGE. In this talk, approach and applications of the methods will be presented.