特別講演
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2A-PL-1445 PDF
Applying Ambient Mass Spectrometry for Characterization and Imaging Neurotransmitters, Metabolites and Drugs on Whole Human Body Skin
Skin, the largest organ of human, plays a key role in protecting the body against environmental pathogens, adjusting body temperature, and assisting removal of metabolites and wastes from the body. Although some skin metabolites such as neurotransmitters are potential biomarkers for diagnosing neurological diseases, current analytical techniques are unable to efficiently characterize them for the difficulty in sampling and their presence in extremely low quantity. In this study, thermal desorption electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) combined with probe sampling was applied to rapidly characterize trace drugs, metabolites, lipids, and neurotransmitters on skin. With the features of simple, rapid and highly sensitive of the ambient ionization mass spectrometric technique, the following studies have been performed: (1) obtaining pharmacokinetic profiles of caffeine on skin to determine individual metabolic rate for precision medicine; (2) characterizing the ingested targeted lung cancer drugs on skin for evaluating the effectiveness of the drugs; (3) studying the influence of different types of physical therapy on the amount of neurotransmitters on skin; (4) constructing 3D molecular imagings of metabolites or drugs to reveal the distribution of them on whole body skin; and (5) identifying the ingested toxins on skin and in various biofluids for emergency management.