オーラルセッション
- 第2日 5月20日（木） 10:00～10:20 A会場（Zoom）
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2A-O6-1000 PDF
大規模コホート研究における血漿メタボロームデータの標準化に向けた取り組み
Metabolomics is an omics study to examine a whole set of small molecules in biological sample and may be able to detect subtle changes in metabolic pathways and the deviation from the homeostasis before the manifestation of phenotypic changes. Nuclear magnetic resonance (NMR)- and mass spectrometry (MS)-based metabolomics has contributed to recent biomarker discovery. However, most case-control studies have analyzed the disease patients, and specificity and sensitivity as biomarkers were not satisfactory for the definition of clinical diagnostics. Therefore, it is necessary to directly compare the absolute concentrations of metabolic profiles with those in a similar healthy control group. The average concentration of plasma metabolites in our large-scale cohort study “Tohoku Medical Megabank Project” by NMR and LC/MS-based metabolomics can be found in the database “Japanese multi omics reference panel, jMorp”, and downloaded with the absolute concentration (μmol/l) of each metabolite in the reference material (the NIST SRM 1950 plasma). The value can be possible to compared when users analyzed the same reference material at their analysis. Therefore, the reference values are essential for evaluating the quality of small molecules in the process of biomarker discovery and establishing the standardized protocol of metabolomics.