- 第1日 5月19日（水） 15:45～16:00 E会場（Zoom）
Native mass spectrometry (Native MS) provides masses of intact biomolecules in combination with nanoelectrospray ionization (nanoESI), which enables ionization of proteins with retaining non-covalent bonds. In this study, we tried to construct the basis for the “single-cell native MS” method that enables analysis of unpurified proteins directly sampled from a single cell. We modified the “Single-cell mass spectrometry” method, which was originally developed by Masujima et al. for metabolomics study1), and applied it to analyze hemoglobin in a single erythrocyte, as the first step for “single-cell native MS”. By optimizing the experimental conditions, we could successfully observe intact hemoglobin ions by native MS using a single erythrocyte.