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第1日　5月19日（水）　16:10～16:30　A会場（Zoom）

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LC-QTOF/MSを用いたグラム陰性腸内細菌のLipid Aの解析

(¹阪大院情報・ ²阪大島津協働研・ ³理研IMS・ ⁴JKiC・ ⁵慶大薬・ ⁶横市大院生命医)
^o岡橋伸幸^1,2,3・ 上田政宏^3,4・ 松田史生^1,2・ 有田誠^3,5,6

A method for the analysis of lipid A, an important component regulating host’s immune function, was developed using LC-QTOF/MS. The sharp peaks of lipid A were observed by employing a metal-free column and high pH basic solvents. This method was applied to the analyses of in vitro cultured gram-negative gut bacteria, E. coli and nine Bacteroidetes strains. The structure of E. coli lipid A was estimated as diphosphoryl hexa-acylated lipid A although many minor and unusual lipid A molecules were also observed in lipid A biosynthetic pathway knockout strains. It was also revealed that Bacteroidetes lipid A was monophosphoryl penta-acylated lipid A but the acyl properties in the main chains were highly heterogeneous.