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1A-O1-1550 PDF
セスキテルペン合成経路解明のためのAspergillus nidulansの揮発性代謝物質の質量分析Ⅹ. AN3280遺伝子発現誘導株
Microbial volatile organic compounds (MVOCs) emitted from Aspergillus nidulans were investigated using Head Space-SPME/GC/MS, and the effects of modified expression of some sesquiterpene biosynthesis genes in the genome of A. nidulans were analyzed. In strain AL37, a part of AN3280 gene upstream was eliminated from LO8910 and a rich alcR protein was added from LO8925. The eliminated region seemed to suppress transcription in the parental strain LO8910. We reported previously that there must be intermediates in the production of (+)-aristolochene from farnesyl diphosphate. However, they were not previously detected. In this study we detected a path from farnesyl diphosphate through the intermediates to (+)-aristlochene in A. nidulans.