日本質量分析学会 第67回質量分析総合討論会

Abstract

Poster Presentations

Day 3, May 17(Fri.)  Room P (Multi-purpose Hall)

Analysis of Substrate Specificity of Aldose Reductase Using LC/MS

(1Mukogawa Women's Univ., 2Mukogawa Women's Univ.)
oShizuyo Horiyama1, Jun Haginaka1,2

Since aldose reductases (ARs) have a lot of families, it is important to develop inhibitors against AR families as drug candidates. Therefore, we tried to clarify the substrate specificity of AKR1B10, one of the AR families, and to evaluate the inhibitory activity of epalrestat, which is one of the inhibitors against AKR1B10, using synthesized substrates. It was found that AKR1B10 selectively reduced the aldehyde form of a substrate, not the keto form. This result suggests that the developed method can be applied to the clarification of the substrate specificity of ARs and the screening of inhibitors against ARs.