Abstract

Poster Presentations

Day 3, May 17（Fri.）　　Room P (Multi-purpose Hall)

3P-24　　PDF

Imaging Mass Spectrometric In-Depth Proteome Analysis of the Kidneys from Rat Model of Diabetic Nephropathy

(¹Doshisha Univ., ²Bruker, ³National Cerebral and Cardiovascular Center)
Yuki Kuzuhara¹, Yume Mukasa¹, ^oTakashi Nirasawa², Ryo Kajita², Hatsue Ueda³, Nobuto Kakuda¹, Masaya Ikegawa¹

Diabetic nephropathy (DN) is a major life-threatening complication of diabetes. Renal lesions affect glomeruli and tubules, but the pathogenesis is not completely defined. Here we have tried to generate in depth proteomic analysis of DN using diabetic animal model, a new inbred rat strain, Spontaneously Diabetic Torii (SDT) fatty rats, which develop hyperglycemia with obesity after 17 weeks of age in comparison with normal control Sprague Dawley (SD) rats of the same age. In this study, we applied matrix-assisted laser desorption/ionization (MALDI) mass imaging mass spectrometry (IMS) for the study of pathological aspects of DN, especially generating in depth proteome analysis by integrating MALDI-IMS and shotgun analysis (timsTOF Pro system).