Abstract

Poster Presentations

Day 3, May 17（Fri.）　　Room P (Multi-purpose Hall)

3P-20　　PDF

Unraveling Pathogenesis of Renal Amyloidosis with an Integrated Workflow of MALDI Imaging Mass Spectrometry and LC-TIMS-MS/MS on Formalin Fixed Renal Biopsy Tissue Section

(¹Doshisha Univ., ²Bruker, ³Necker-Enfants Malads Hospital, ⁴Georges Pompidou European Hospital, ⁵National Cerebral and Cardiovascular Center)
^oYume Mukasa¹, Takashi Nirasawa², Ryo Kajita², Marion Rabant³, Huyen Duong Van Jean-Paul³, Bruneval Patrick⁴, Hatsue Ueda⁵, Nobuto Kakuda¹, Masaya Ikegawa¹

Here we have established an integrated workflow of matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) in combination with shotgun proteomics using liquid chromatography, trapped ion mobility and mass spectrometry (LC-TIMS-MS/MS). An analytical technique was developed and evaluated through a combination of specific sample preparation/extraction protocols based on the same formalin-fixed tissue section of renal biopsy tissue samples. Our strategy succeeded in the discrimination of subgroups of renal amyloidosis such as immunoglobulin light chain (AL) and serum amyloid A (SAA) type. With the current strategy, we have detected some of the proteins that may provide insights into the pathogenic process of amyloid formation in human kidneys.