日本質量分析学会 第67回質量分析総合討論会

Abstract

Poster Presentations

Day 3, May 17(Fri.)  Room P (Multi-purpose Hall)

Dissecting Pathogenesis of Dilated Cardiomyopathy (DCM) on J2N-k Hamster Model Using MALDI-Imaging Mass Spectrometry in Combination with Shotgun Proteomics

(1Doshisha Univ., 2Kyoto Univ., 3Bruker, 4National Cerebral and Cardiovascular Center)
oInori Shintani1, Takashi Tsuji2, Mizuki Ishida1, Takashi Nirasawa3, Nobuto Kakuda1, Hatsue Ueda4, Hidetoshi Masumoto2, Kenji Minatoya2, Masaya Ikegawa1

Dilated Cardiomyopathy (DCM) has an extremely poor prognosis. To investigate the pathogenesis of DCM, we performed global proteomic analysis of myocardial tissues from J2N-k cardiomyopathic hamsters. In the current study, we tried to integrate in situ proteomics for cardiac tissues from hamsters by integrating Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) and LC-trapped ion mobility spectrometry (TIMS)- MS/MS . Histological examinations as well as serial cardiac tissue sections from hamsters at each weeks of age were analyzed with rapiflex tissue typer and tims TOF Pro with nanoElute. MALDI-IMS in combination with a shotgun proteomic analysis is a useful approach in analyzing cardiomyopathic tissues from DCM animal model.