Abstract

Oral Sessions

Day 3, May 17（Fri.）　16:05-16:25　Room E (201)

3E-O2-1605　　PDF

Novel Analytical Method for Large-Scale Metabolomics by Mixed-Mode Hydrophilic Interaction/Anion-Exchange Liquid Chromatography Tandem Mass Spectrometry

(¹Kyushu Univ., ²Hitachi Chemical Techno)
Kohta Nakatani¹, ^oYoshihiro Izumi¹, Masatomo Takahashi¹, Keita Sakurai², Michio Butsugan², Takeshi Bamba¹

Essential metabolic pathways to maintain biological activities include glycolysis, pentose phosphate pathway, tricarboxylic acid cycle, amino acid, and nucleic acid metabolism. Almost all intermediates of these metabolic pathways are polar and ionic compounds such as amino acids, nucleic acid constituents, organic acids, sugar phosphates, coenzyme, etc. Therefore, a profiling for these hydrophilic metabolites provides valuable insight towards understanding metabolic regulation. However, since these hydrophilic metabolites have a wide variety of physicochemical properties, it is difficult to comprehensively measure them with a single analytical run. In the present study, we have developed a highly sensitive and practical analytical method that provides wide metabolite coverage using mixed-mode hydrophilic interaction/anion exchange liquid chromatography tandem mass spectrometry (HILIC/AEX/MS/MS) with a novel polymer-based amino column.