日本質量分析学会 第67回質量分析総合討論会

Abstract

Oral Sessions

Day 3, May 17(Fri.) 15:05-15:25 Room C (101)

Analysis for Structural Change of NCP by Acetylation Using Mass Spectrometry

(1Hiroshima Univ., 2Yokohama City Univ., 3Waseda Univ., 4Univ. Tokyo)
oKazumi Saikusa1,2, Haruna Hidaka1, Hiroki Shinya1, Nanako Azegami2, Daiki Kato3, Hitoshi Kurumizaka3,4, Shunsuke Izumi1, Satoko Akashi2

Post-translational modifications (PTMs) of histone tails in nucleosome core particle (NCP), such as histone acetylation, play crucial roles in regulating gene expression. In this study, to understand the regulation mechanism caused by the structural change of NCP by acetylation, two NCPs (NCP with Widom 601 DNA and α-satellite DNA) were reconstituted in vitro, acetylated with histone acetyltransferase, and characterized using native mass spectrometry. In the case of NCP with 601 DNA, artificially prepared tight-wrapping NCP, no change was detected in the mass spectra with/without acetylation. On the other hand, the peaks of dissociated DNA were significantly observed in the mass spectra of the acetylated NCP with α-satellite DNA in 1 M NH4OAc solutions. It was suggested that structural stability in high salt concentrations of the acetylated NCP with α-satellite DNA was lower than that of the unmodified NCP. Additionally, it will be discussed the relationship between the structural stability of acetylated NCP and epigenetic regulation.