日本質量分析学会 第67回質量分析総合討論会

Abstract

Oral Sessions

Day 3, May 17(Fri.) 10:10-10:30 Room C (101)

A High Throughput Mass Spectrometry Plate Reader

(Sciex)
oTetsuo Kokaji

Label-free LC/MS based screening technology is routinely used in pharmaceutical industries for hit discovery and various ADME profiling applications. Although the current analysis speed of <30 seconds per sample is quite promising, it still cannot match the throughput provided by plate-reader based HTS platforms. Acoustic droplet ejection (ADE) is a droplet transfer technology capable of high speed, reproducibility and absolute accuracy. In this work, we successfully couple ADE and the standard ESI ion source of a mass spectrometer with the open-port probe (OPP) sampling interface. Screening speeds as fast as 0.4 seconds-per-sample are demonstrated with superb sensitivity, great reproducibility even without internal standard, good quantitation capability, no matrix effect, and broad compound coverage.