日本質量分析学会 第67回質量分析総合討論会

Abstract

Oral Sessions

Day 3, May 17(Fri.) 15:45-16:05 Room B (102)

Quantitative Comparison of Amines in WKY and SHRSP Rat Brain Using MALDI ImagingMS “Triple Spray" Method

(1Astellas Pharma, 2Sekisui Med.)
oMasashi Hiramoto1, Toshimasa Ito2, Satoshi Ito2, Tsutomu Negama2

MALDI-Imaging Mass Spectrometry can visually elucidate the distribution of substances in biological sample. In case of using this technique for application in drug development, it requires quantitativity. However, currently there is no effective quantitative method. In order to solve this problems, we developed the Triple Spray method using derivatization reagent mTRAQ. This method enables us to quantify the amine distribution by normalizing the amine specific matrix effect. This allows us to get a better representation of the distribution and quantitate each target compound. In this study, we compared the distribution of neurotransmitter amino acids in WKY rat brain and SHRSP rat brain quantitatively.