Oral Sessions (Day1, Day2, Day3)
Poster Presentations
(Day1, Day2, Day3)
Oral Sessions
- Day 3, May 17（Fri.） 15:25-15:45 Room B (102)
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3B-O2-1525 PDF
MALDI Imaging Mass Spectrometry for N-Glycans on NASH Liver FFPE Tissue Sections through Sialic Acid Derivatization
Glycans play important roles in the physiological process and are drawing attention as biomarkers. Glycans can be detected by mass spectrometer, especially recently matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) has enabled the visualization of the distribution of glycans on tissues. However, MALDI-IMS of glycans with sialic acids (sialo-glycans) is still challenges because of the weakness of the detection signal due to the carboxyl group of the sialic acids. In this study, to detect sialo-glycans with high sensitivity by MALDI-IMS, we focused on derivatization of sialic acids. We investigated the reaction condition of amidation and developed a sialic acid derivatization by benzylamidation which is more sensitive than conventional method of amidation. Furthermore, we transferred this method onto formalin-fixed paraffin-embedded (FFPE) liver tissue of normal and non-alcoholic steatohepatitis (NASH) model mouse for MALDI-IMS.